The newest addition to the BIF infrastructure is the Zeiss Total Internal Reflection Fluorescence, or TIRF, microscope. This microscope is designed to examine cellular structure located very near the surface of the cell (interface between the cell and the coverslip). Equipped with a CO2 chamber and incubator, this system is ideal for mammalian cell biology projects. This system is also capable of performing FRAP imaging and will detect the faintest signals with the new ORCA-Flash 4.0 sCMOS camera.